Thanks. It's worth stating that the aperture setting on the lens is only true for the lens mounted normally on the camera and focused at infinity. If the lens is reversed the aperture setting of f/8 probably translates to something like f/22 once you take extension and the different configuration into account. That's well into diffraction territory, but obviously good results can still be obtained.
I almost never use extension tubes, as they degrade things rather quickly IMO.
I am not much at calculating diffraction-per-aperture, reversed, but rather just go by my eye.
My eye tells me that, with the 28mm Ai-S, f/8-f/11 apertures, stacked, give me buttery-smooth images (if I keep the ISO down) ... but at f/16+ I begin to notice unacceptable diffraction.
Focus stacking explains why you were able to get so much DOF. That's really good use of stacking as it looks natural, if stacking is overdone the picture is too sharp front to back and looks flat. It's amazing the spiders were still long enough for you do do it.
I agree; I don't like "everything in-focus" in most macro shots either, as the images look more like CGI graphics than artistic macro shots. I much prefer the fore- and aft-bokeh to macro imagery.
Regarding their standing still, these particular species are "freeze and ambush" spiders, so they're great for stacking. Once they settle down, they just sit there ... "waiting" ... It is only the species which are proactive hunters that are almost impossible to stack, but these guys, above, are a piece of cake

As for "focusing" the reversed lens, if the lens is unit-focusing (such as the AIS 20/3.5) it makes no difference optically. If you set the focus ring to infinity you gain a few mm extra working distance because the rear element is further to the rear (or front since it's reversed...) On the other hand if you focus close, the rear element retracts into the barrel so you lose a bit of working distance, but the rear element is better protected from knocks.
If the lens has floating elements (such as the AIS 28/2.8 ) then the setting of the focus ring does make a difference optically. With the lens reversed, if the subject - sensor distance is 0.3m, then the lens should be focused to 0.3m, so the optics are properly configured for that distance.
Interesting distinction, thanks.
These were taken with the Ai-S 28mm, and I like the results better with the min. focus distance set.
Jack