Bob
the statistics of your graph look quite appealing. Made me poke around the internet to find out more about FocusTune.
Do I understand the procedure correctly: Step1, You set up the LenAlign target, which is slightly more tricky than just a basically planar target. Step 2, you shoot in rapid succession jacking the the AF Adjust value from time to time. Step 3, you feed the whole bunch of jpg to the program FocusTune which analyses each image and determines the amount of front and back focus automatically. That task is more challenging than just grading sharpness: bravo for Michael Tapes, its software designer ! The only minor problem I could see is the influence of field curvature on finding the position of the zero line: the front/back focus measurement area is offset from the AF target.
I did not see a specific recommendation for the optimal Adjust value. In any case its about -2. Congrats for the lens - camera combo, you have to test very carefully, as you did, to realise such a small potential for improvement.
What you could do, is to reduce the number of images for the outer values of AF Adj, also by bigger steps. But then gain better info on the zero line crossing by doing more shots in the range of interest. Suggestion: do 10 shots for each Adj value from -4 to +1 for this combo.
As out in the wild you may focus coming in from near, or from far mostly out of your control, you might be interested to find out with the improved statistics if there would be a different Adjust for the two sides of approach.
My feeling is that a contrast maximising procedure, like mine or FoCal, tends to be more disturbed by the statistics of the measurements.