Thank you Bjorn... i posted 275 images above.. The Mitutoyo M-PLan 2x objective has a DoF of 0.091mm so given the 12mm or so of bee (from memory) this represents almost 2x oversampling.. i probably could have gotten away with 130 images.... so my step size was probably half the DoF....
Added Note... I have found that oversampling helps reduce artifacts in the composite image. This image required very little retouching.. I normally use both Pmax and Dmap algorithms, setting the threshold on Dmap and retouching with Pmax.