Images > Nature, Flora, Fauna & Landscapes

Bee

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bobfriedman:
Nikon D800E ,Mitutoyo M Plan APO 2x NA 0.055 200/0
275 stack

Bjørn Rørslett:
The overall image clarity is excellent. I assume this results from focus stacking and am curious as to how many images were required and what focus step size you sued.

bobfriedman:
Thank you Bjorn... i posted 275 images above..  The Mitutoyo M-PLan 2x objective has a DoF of 0.091mm so given the 12mm or so of bee (from memory) this represents almost 2x oversampling.. i probably could have gotten away with 130 images.... so my step size was probably half the DoF....

Added Note... I have found that oversampling helps reduce artifacts in the composite image. This image required very little retouching.. I normally use both Pmax and Dmap algorithms, setting the threshold on Dmap and retouching with Pmax.

bobfriedman:
Nikon D800E ,Mitutoyo M Plan APO 5x NA 0.14 200/0
275 stack

bobfriedman:
Nikon D800E ,Mitutoyo M Plan APO 20x NA 0.42 200/0
275 stack

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