NikonGear'23

Images => Nature, Flora, Fauna & Landscapes => Topic started by: bobfriedman on July 19, 2015, 23:44:11

Title: FLY
Post by: bobfriedman on July 19, 2015, 23:44:11
this was a rather large one..

Nikon D800E ,Mitutoyo M Plan APO 5x NA 0.14 200/0
180 stack iso100, RAYNOX TUBE 170mm bellows extension, 5mm objective extension - aprox 3.9x
(http://www.pbase.com/image/160783131/original.jpg)

Nikon D800E ,Mitutoyo M Plan APO 20x NA 0.42 200/0
100 stack iso100
(http://www.pbase.com/bobfriedman/image/160781695/original.jpg)
Title: Re: FLY
Post by: elsa hoffmann on July 19, 2015, 23:47:22
the pose makes it look like a dainty lady fly

very well done as usual Bob
Title: Re: FLY
Post by: bobfriedman on July 19, 2015, 23:54:48
thx... this fly was 16.5mm in length.. not small.
Title: Re: FLY
Post by: bobfriedman on July 19, 2015, 23:56:33
Nikon D800E ,Mitutoyo M Plan APO 5x NA 0.14 200/0
140 stack iso100, RAYNOX TUBE 170mm bellows extension, 5mm objective extension - aprox 3.9x
(http://www.pbase.com/bobfriedman/image/160781298/original.jpg)
Title: Re: FLY
Post by: bobfriedman on July 20, 2015, 03:02:27

Nikon D800E ,Mitutoyo M Plan APO 10x NA 0.28 200/0
190 stack iso100
(http://www.photomacrography.net/forum/userpix/743_20150719190646ZSweb_1.jpg)
Title: Re: FLY
Post by: armando_m on July 20, 2015, 04:01:01
wow a blond fly! do you think she bleached ?  :)

Impressive detail !
Title: Re: FLY
Post by: bobfriedman on July 21, 2015, 03:55:23
Nikon D800E ,Mitutoyo M Plan APO 20x NA 0.42 200/0
190 stack iso100, Ai-S Micro-Nikkor 200mm f/4 IF relay
(http://www.pbase.com/bobfriedman/image/160797835/original.jpg)
Title: Re: FLY
Post by: Richard A on July 21, 2015, 08:23:30
This is truly amazing. I thought that such detail was only possible with an electron scanning microscope, not with traditional optics!
Title: Re: FLY
Post by: Akira on July 21, 2015, 13:20:49
Wow, the 20x images are stunning!  Is the rainbow color on the compound eyes fixed, or caused by iridescence or some sort of prism effect?
Title: Re: FLY
Post by: bobfriedman on July 21, 2015, 19:25:42
Wow, the 20x images are stunning!  Is the rainbow color on the compound eyes fixed, or caused by iridescence or some sort of prism effect?

It is fixed, you can see it with your eyes. I believe it fades as the eyes dry out, so it is not fixed forever.

I really don't know why it is there and I was surprised to see it.
Title: Re: FLY
Post by: elsa hoffmann on July 21, 2015, 20:12:00
Unbelievably great
Title: Re: FLY
Post by: Peter Connan on July 21, 2015, 20:28:46
That is an insane amount of detail you have managed to capture!
Title: Re: FLY
Post by: Bjørn Rørslett on July 21, 2015, 20:34:36
Bob, I was intrigued by the apparent added amount of detail when the 200 Micro is used as a relay lens. The early 200 Micro-Nikkor was, as I'm sure you are aware of, regarded as the least satisfying of the trio 55-105-200. However, no one would believe this based upon the evidence presented here. Thus, do you have to remove lots of CA and other artefacts, or are the image (in principle) straight off the camera?
Title: Re: FLY
Post by: bobfriedman on July 21, 2015, 21:18:37
The images are processed using contrast, saturation and unshared masking. There is no CA removal per se. In particular, I use Octave unsharp masking.

The AiS Micro-Nikkor 200/4 is a big improvement over the old Nikkor 200/4 presumably due to the improved coatings. I also got much better results using the reversed Raynox lens which requires a significant number of extension tubes which I flocked to mitigate internal reflections.

The problem here was the "hot spot" in the first 20x image which may be due to direct reflection from the eyes, since change the relay lens to the Micro-Nikkor did not eliminate the hot spot.

I no longer use the older Nikkor 200/4. The added value of the Raynox lens with extension tubes is that I can adjust the magnigfication by reducing the tube length and hence get intermediate magnifications from the objective ratings. If you notice the first image is at 3.9x using a 5x objective
Title: Re: FLY
Post by: bobfriedman on July 21, 2015, 21:26:44
The Raynox tube relay lens is described here http://nikongear.net/revival/index.php?topic=71.0
Title: Re: FLY
Post by: Bjørn Rørslett on July 21, 2015, 21:42:04
Hot spots can be tricky to reduce if at all possible. UV (and IR) photographers are only too well familiar with this problem.

Adding black flocking material inside tubes, or making stray light traps, can sometimes alleviate the issue. The old M/M2 tubes for the 55 Micro-Nikkor had a rectangular cut-out at their rear, which can be removed and used elsewhere. It certainly solved a couple of my hot spot issues.

An old trick is surrounding the subject itself with black velvet or similar material to cut off stray light just outside the field of view of the taking lens.
Title: Re: FLY
Post by: bobfriedman on July 21, 2015, 22:16:29
Problem is that I illuminate the subject through a diffusing styrofoam cup. Using the velvet flocking would not be an option in this case but it is a good idea in general.

I also wrap the metallic objective with black absorber to minimize stray reflections.
Title: Re: FLY
Post by: Bjørn Rørslett on July 21, 2015, 22:34:55
You could still surround the fly with black velvet ? The basic idea is to cut down on light reflected from outside the field of view that still manages to contaminate your subject.
Title: Re: FLY
Post by: bobfriedman on July 21, 2015, 23:45:58
i will think of way i can do that... may not be all that easy.. but if the dominant reflections are coming from the eyes, it may not do much good.
Title: Re: FLY
Post by: Bjørn Rørslett on July 21, 2015, 23:48:14
Won't hurt to try.
Title: Re: FLY
Post by: Jørgen Ramskov on July 31, 2015, 16:32:36
I'm out of words. Incredible details, truly impressive work.
Title: Re: FLY
Post by: bobfriedman on July 31, 2015, 22:17:55
i am wondering now if the hotspot was the reflection of the lens objective